Activity-induced gene expression and long-range enhancer-promoter contacts in cohesin-deficient neurons (Ribo Tag)

RNA-seq of ribosome associated mRNA from Rad21lox/lox;NexCre/+;RiboTag+ and Rad21+/+;NexCre/+;RiboTag+ dissociated cortical neurons at Day In Vitro (DIV10) Overall design: RNA-Seq was performed on mRNA from immunoprecipitated polysomes of DIV10 cortical dissociated neurons. Three replicates of Rad21lox/lox;NexCre/+;RiboTag+ and three replicates of Rad21+/+;NexCre/+;RiboTag+ DIV10 cortical dissociated neurons were used. Neurons were heterozygous for the Rpl22-HA (RiboTag) allele, which expresses an HA-tagged ribosomal protein specifically in Cre-expressing neurons.