Jennifer Bestman, Holly Cline (2011) CIL:36855, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #2 (10 minutes) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The images were acquired with 10 minute intervals and show morphological plasticity of the dendritic arbor with branch additions and retractions.

These cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

Images were acquired every 10 minutes for 1 hour. After 60 minutes, the animal was placed in a 50 microMolar anisomycin solution for ~1.5 hours. A second time lapse series (10 minute interval for 60 minutes) was then acquired.

本图组中包含的时间序列快照之第二帧（间隔10分钟）。此帧展示了一只白化非洲爪蟾（Xenopus laevis）中枢神经系统视顶盖中的神经元。动物处于47期发育阶段。通过10分钟的时间间隔采集到的图像系列，揭示了树突丛的形态可塑性，包括分支的增加和回缩。这些细胞在首次图像采集前约24小时，通过原位电穿孔技术转染编码eYFP的质粒DNA。完整的活体蝌蚪在麻醉状态下，置于玻片下，并通过定制的双光子显微镜直接穿过其透明的皮肤进行成像。图像每隔10分钟采集一次，持续1小时。60分钟后，动物被置于50微摩尔异霉素溶液中处理约1.5小时。随后，又进行了一次时间序列快照的采集（持续60分钟，时间间隔为10分钟）。