Proteome and phosphoproteome data of peanut seedlings under salt stress

Soil salinity is one of the major factors that limits area of cultivable land and yield potential of crops. Considered as a moderately salt sensitive economically-important crop, biological processes involved in salt stress response in peanut remain elusive. Publishing of the genomic sequence of cultivar peanut represent a new opportunity for further research. ABA INSENSITIVE 4 (ABI4) is an evolutionary conserved transcription factor. Mutation of ABI4 in Arabidopsis enhanced salt tolerance of seedlings significantly by targeting and down-regulating of HKT1;1. However, few achievements were reported in other aspects. In this study, AhABI4s in peanut were first cloned and silenced via virus-induced gene silencing method. Phenotype analysis showed that down-regulation of AhABI4s enhanced salt tolerance of peanut significantly. According to proteome and phosphoproteome analysis, expression of 1,900 proteins and 2,620 phosphorylation sites were predicted to be affected by silencing of AhABI4s under salt stress. Proteins with ion transporting activity were enriched by GO analysis. Further analysis showed that, 31 proteins may participate in homeostasis maintain of Na+, K+, Ca2+, H+ and Cl- in plant cells. Combined transcriptome and proteome analysis indicated that 63 genes may regulated by AhABI4s directly and 7 of them, ABI5-like5, RABA1f, SCAMP3, PK, RP-S26e, HSP70, cysteine proteinase inhibitor, were able to interact with the predicted ion transporters. Silencing of AhABI4s altered down-stream protein-protein interaction network to regulate expression/phosphorylation level of ion transporters, and finally influence homeostasis under salt stress. This work provides novel insights for salt response mechanism research and offer important evidence for protein function study.