Efficient gene knockout and genetic interaction screening using the in4mer CRISPR/Cas12a multiplex knockout platform

Figure 1: Meta-analysis of synthetic lethality screens.Figure 2: Multiplexing beyond 2 guides with enAsCas12a.Figure 3: In4mer platform for whole-genome screeningFigure 4: Prototype and Inzolia screensFigure 5: Synthetic chemogenetic interactionsFigure 6: Library size comparisonSupplementary Table 1: dLFC and Cohen's D for five published paralog studiesSupplementary Table 2: 388 Identified synthetic lethals, 26 synthetic lethals and gold standard setSupplementary Table 3: Raw read counts for 7mer screensSupplementary Table 4: Prototype library sequenceSupplementary Table 5: Inzolia library sequenceSupplementary Table 6: Raw read counts for both Prototype and Inzolia library screensSupplementary Table 7: Log fold change by gene for both Prototype and Inzolia library screensSupplementary Table 8: DrugZ score for MELJUSO screen with MEK inhibitor selumetinib Supplementary Table 9: GSEA result for MELJUSO screen with MEK inhibitor selumetinib