Additional file 10 of Transcriptome analyses of liver in newly-hatched chicks during the metabolic perturbation of fasting and re-feeding reveals THRSPA as the key lipogenic transcription factor

Additional file 10: Information on primers used for qRT-PCR verification of differential gene expression. A Microsoft Excel file containing a single worksheet “Primers qRT-PCR Assay”. This table provides information on the design of qRT-PCR primers for 20 chicken genes. The Roslin Institute Gallus gallus (RIGG) oligo ID number, NCBI Entrez Gene ID, gene symbol, gene description, and the 5′-3′ sequence for forward and reverse primers used for qRT-PCR analysis are provided. Although four “invariant genes” were included, only two genes were acceptable by qBase software for use in normalization of qRT-PCR expression levels.

附加文件10：用于差异基因表达实时定量逆转录聚合酶链反应（qRT-PCR）验证的引物信息。该文件为Microsoft Excel格式，仅包含一个名为"Primers qRT-PCR Assay"的工作表。本表格提供了20个鸡基因的qRT-PCR引物设计相关信息，具体包括罗斯林研究所家鸡（Roslin Institute Gallus gallus, RIGG）寡核苷酸编号、NCBI Entrez基因ID、基因符号、基因描述，以及用于qRT-PCR分析的正向与反向引物的5′→3′序列。尽管共纳入了4个"持家基因（invariant genes）"，但经qBase软件校验，仅2个基因可用于qRT-PCR表达水平的归一化分析。