Downregulation of ADAM10 drives differentiation of radial glial cells in the ventral spinal cord.

After electroporation at E4, transverse sections were used for detection at E6. The transfected cells are marked by green fluorescence and the control morpholinos (Fluo-m) and the untransfected side (left side) serve as controls. The immune reactive cells are stained by red. Cell nuclei are labeled by DAPI (blue). Immunohistochemistry was performed in adjacent sections with antibodies against Nestin (A–H) and vimentin (3CB2; I–M), respectively. Arrows in A–D indicate transfected regions (green) and in E–K the inhibition of nestin (E–H) and vimentin (I–K) in the transfected region. Noted that no change of viementin expression in the transfected region above the floor plate is found (arrows in L, M). Scale bar, 200 µm in (A) for (A–K).