Additional file 2 of A successful defence strategy in grapevine cultivar ‘Tocai friulano’ provides compartmentation of grapevine Flavescence dorée phytoplasma

Additional file 2: log2 relative transcript level of selected categories of genes obtained from 1-year-old canes grown on symptomatic (S, AS and AA) and asymptomatic (A) 2-year-old arms of Flavescence dorée infected 'Tocai friulano' plants or from healthy plants (H). For each gene the relative expression was calculated by setting a value of 1 as the lowest value among S, AS, AA, A or H samples. Samples were collected for two years (2020 and 2021) during September and November. Normalisation of transcriptional levels were performed using GAPDH and COX as endogenous controls. Significant differences (P ≤ 0.05) among treatments are indicated by different letters, according to parametric one-way ANOVA using Student Newman Keuls test.

附加文件2：收录了选定基因类别的log₂相对转录水平，其材料来源分为两类：一类为感染葡萄金黄化病（Flavescence dorée）的托凯·弗里亚诺（Tocai friulano）植株的带症状（S、AS、AA）、无症状（A）二年生结果母枝上所生长的一年生成熟枝蔓，另一类为健康植株（H）。针对每个基因，以S、AS、AA、A或H各组样本中的最低表达量值为参照并设为1，以此计算相对表达量。样本于2020年和2021年的9月及11月采集。转录水平的归一化分析以甘油醛-3-磷酸脱氢酶（GAPDH）和细胞色素C氧化酶（COX）作为内参基因完成。根据参数单因素方差分析（one-way ANOVA）结合Student Newman Keuls检验，不同字母标注表示各组处理间存在P≤0.05的显著差异。