Diversity and ecology of green microalgae in marine systems: an overview based on 18S rRNA sequences.Perspectives in Phycology. Supplementary material. DOI: 10.1127/pip/2016/0059

<b>1 and 2. Data S1</b>. Fasta and taxo files of annoted Chlorophyta GenBank sequences. Two files containing sequences in fasta format and their annotated taxonomy. These files can be used to assign metabarcoding data using software such as mothur or Qiime.<br><br><b>3. Data S2</b>. Chlorophyta sequences metadata.<br><br><b>4. Data S3</b>. Fasta file of the reference MAFFT alignment for the phylogenetic tree (Fig.1). 132 rRNA 18S reference sequences (Supplementary Table S1) provide a length alignment of 1752 bp. Alignment was stripped at 50% (columns of the alignment counting more than 50% gaps were deleted).<br><br>

**1、2. 补充数据集S1（Data S1）**：包含经注释的绿藻门（Chlorophyta）GenBank序列的FASTA格式（Fasta）文件与分类学文件。该数据集内含两个文件，分别为FASTA格式的序列文件及其附带注释的分类学信息文件，可借助mothur、Qiime等软件完成元条形码测序（metabarcoding）数据的分类赋值工作。

**3. 补充数据集S2（Data S2）**：绿藻门序列元数据。

**4. 补充数据集S3（Data S3）**：用于生成系统发育树（图1）的MAFFT多序列比对参考序列FASTA文件。其中收录132条18S核糖体RNA（18S rRNA）参考序列（详见补充表S1），比对序列的总长度为1752 bp。本次比对以50%空位占比为阈值进行了过滤处理，即移除所有空位占比超过50%的比对列。