Activity of protease isoenzymes against various protein and chromogenic substrates.

†Specific activity of partially deglycosylasted protease isoenzymes; ND: not determined.#Incubated for 180 min at 37°C.Substrate(s) for 1trypsin, 2,3thrombin, 4plasmin, 5FXa.Incubation was carried out with 1% (w/v) protein substrate at pH 8.0, 37°C for 90 min or #180 min. For amidolytic activity assay, protease was incubated with 0.2 mM chromogenic substrate for 10 min at 37°C. Values are mean±SD of triplicate determinations. Values in the same row with different subscripts (a–e) are significantly different (P