PI3K activity is required for induction of cerebellar LTP.

A) Application of the irreversible PI3K inhibitor wortmannin (1 µM; n = 9 cells) to the bath from 10 min before until 10 min after 1 Hz stimulation for 5 min (arrow) reduces the amplitude and duration of LTP. B) Application of the reversible PI3K inhibitor LY294002 (50 µM; n = 8) to the bath from 10 min before until 10 min after 1 Hz stimulation for 5 min (arrow) blocks LTP induction. Data are mean ± s.e.m. Single sample t test was used to test for a significant increase in mean amplitude of five consecutive pulses at 10 min and 30 min post LTP-evoking tetanus; p values were as indicated. Traces are averages of 5 consecutive EPSCs from a representative cell before, 10 min and 30 min after 1 Hz stimulation. Grey traces reproduce EPSC before 1 Hz for comparison. C) Addition of wortmannin to the bath solution for 10 min before and throughout application of 10 µM DEA/NO blocks LTP. Data are mean ± s.e.m. of 6 cells. ns = mean amplitude is not significantly different from 1 (p>0.05, single sample t test). Traces are average of 5 consecutive EPSCs from a representative cell before, 10 min and 30 min after DEA/NO application. Grey traces reproduce EPSC before DEA/NO for comparison.