Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain - WT-WT - 5169_5208

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.

本研究成对评估了陌生个体间的社会互动与交流行为。针对社交行为与超声通讯（ultrasonic vocalizations, USVs）的录制工作，我们仅选取个体对作为研究对象，原因在于当动物近距离互动时，当前无法分辨超声发声（USVs）的发声个体。因此，我们对来自不同饲养笼、年龄一致（14至20周龄）且基因型相同的两只陌生个体之间的无干扰成对互动，开展了为期47小时（两天两夜）的录制。为此，我们将活体小鼠追踪系统（Live Mouse Tracker，插件版本931）与一台CM16/CMPA型麦克风（德国格利尼克市Avisoft Bioacoustics公司出品）相连，该麦克风外接Avisoft Ultrasound Gate 416设备，其参数设置为：采样率300 kHz，格式为16位；触发阈值：该通道电平；预触发时长1秒；保持时长1秒；有效时长>0.005秒；触发事件：25-125 kHz频段内能量占比2%且熵值<50%；该设备由德国格利尼克市Avisoft Bioacoustics公司提供。活体小鼠追踪系统（LMT）与Avisoft设备的同步方案参照de Chaumont等人2021年发表的研究规程。每份上传数据均包含经处理且完成超声发声（USVs）同步的活体小鼠追踪系统（LMT）SQLite数据库，以及由Avisoft设备自动录制的全部超声发声序列ZIP压缩包。