Tumor Necrosis Factor Alpha Regulates Skeletal Myogenesis by Inhibiting SP1 Interaction with cis-Acting Regulatory Elements within the Fbxl2 Gene Promoter

Using NGS technology we describe the transcriptomic effecs of TNFa stimulation on skeletal myogenesis and, through gene sliencing techniques, compare the effects of TNFa with the loss of a critical cell cycle regulator, Fbxl2, on the process of myogenic proliferation and differentiation. Overall design: RNA sequencing was performed on C2C12 cells during the stage of myoblast proliferation (proliferation), at 48 h post induction of differentiation (control), in the presence of 10 ng/ml TNFa stimulation (TNF) at 48 h, and after Fbxl2 gene silencing at 48 h post induction of differentiation.