Genetic selection of growth-inhibitory sequences in mammalian cells.
收藏PubMed Central1994-12-20 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC45476/
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资源简介：
To assess the role of mitogenically activated genes in the control of cell proliferation, we have taken a genetic approach based on the premise that blocking the function of an essential gene should lead to growth inhibition. Using a newly developed selection procedure, we isolated growth-inhibitory sequences from a pool of random cDNA fragments of 19 growth-related genes associated with the G0/G1 transition. These sequences encode potential dominant negative variants of c-Fos, JunB, and p44MAPK that may interfere with their growth-related functions. We anticipate that this procedure, which allows for the selection of sequences that cause a growth-inhibition phenotype, may have broad applications in the identification and analysis of genes that regulate cell growth. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1994-12-20
