Transcriptomic analysis of Pinellia ternata (Thunb.) Breit T2 plus line provides insights in host responses resist Pectobacterium carotovorum infection

Transcriptome is used to determine the induction response of <i>Pinellia ternata (Thunb.) Breit</i> T2 plus line (abbreviated as <i>PT2P</i> line) infected with <i>Pectobacterium carotovorum</i>. The main objective of the study was to deal with the transcriptome database of <i>PT2P</i> line resistance to soft rot pathogens to provide a new perspective for identifying the resistance-related genes and understanding the molecular mechanism. Results indicated that water soaking and tissue collapse started at 20 h after <i>PT2P</i> line was infected by <i>P. carotovorum</i>. A total of 1360 and 5768 differentially expressed genes (DEGs) were identified at 0 h and 20 h, respectively. After 20 h of infection, growth and development-related pathways were inhibited. Meanwhile, DEGs were promoted the colonization of <i>P. carotovorum</i> pathogens in specific cell wall modification processes at the early infected stage. A shift to a defensive response was triggered at 0 h. A large number of DEGs were mainly up-controlled at 20 h and were substantially used in the pathogen recognition and the introduction of signal transformation cascades, secondary metabolites biosynthesis, pathogenic proteins activation, transcription aspects and numerous transporters. Furthermore, our data provided novel insights into the transcript reprogramming of <i>PT2P</i> line in response to <i>P. carotovorum</i> infestation.

本研究借助转录组（Transcriptome）测序技术，探究半夏（*Pinellia ternata (Thunb.) Breit*）T2加系（缩写为PT2P品系）感染胡萝卜软腐果胶杆菌（*Pectobacterium carotovorum*）后的诱导响应。本研究的核心目标为构建PT2P品系抗软腐病病原体的转录组数据库，为鉴定抗病相关基因、解析其分子调控机制提供全新视角。研究结果显示，PT2P品系被胡萝卜软腐果胶杆菌侵染后，20小时开始出现水渍状病斑与组织溃陷症状。分别在侵染后0小时与20小时鉴定得到1360个和5768个差异表达基因（differentially expressed genes, DEGs）。侵染20小时后，与生长发育相关的通路受到显著抑制；同时，侵染早期的差异表达基因可促进胡萝卜软腐果胶杆菌在特定细胞壁修饰过程中的定殖。侵染0小时即触发防御响应的转换，侵染后20小时，大量差异表达基因呈现上调表达，主要参与病原体识别、信号转导级联反应、次生代谢产物生物合成、致病蛋白激活、转录调控以及多种转运蛋白相关过程。本研究数据为解析PT2P品系应对胡萝卜软腐果胶杆菌侵染的转录组重编程机制提供了全新见解。