Innate triggering and antiviral effector functions of activin A

5A:2^dCt GAPDH-INHBA expression following transfection of human PBMC (peripheral blood mononuclear cells) with IVT-RNA or poly(I:C) at two doses

5B:2^dCt GAPDH-MX1 expression following transfection of human PBMC with IVT-RNA or poly(I:C) at two doses
5C:2^dCt GAPDH-IFI6 expression following transfection of human PBMC with IVT-RNA or poly(I:C) at two doses
5D:2^dCt GAPDH-INHBA expression following exposure of human PBMC to the imidazoquinolones R848 and R837
5E:2^dCt GAPDH-MX1 expression following exposure of human PBMC to the imidazoquinolones R848 and R837
5F:2^dCt GAPDH-INHBA expression following transfection of human PBMC with ssRNA40 LyoVec (TLR8 agonist)

5G:2^dCt GAPDH-IFI6 expression following transfection of human PBMC with ssRNA40 LyoVec (TLR8 agonist)
6A:2^dCt GAPDH-INHBA at 48 hours post-infection in A549.gfp cells infected with increasing titres of Sendai virus (SeV) compared to controls
6B:2^dCt GAPDH-MX1 at 48 hours post-infection in A549.gfp cells infected with increasing titres of Sendai virus (SeV) compared to controls
6C:2^dCt GAPDH-INHBA expression in HuH7 (RIG-I replete) versus HuH7.5 (RIG-I deficient) cells following SeV infection at various multiplicities, assayed at 48 hours post-infection.
6D:2^dCt GAPDH-MX1 expression in HuH7 (RIG-I
replete) versus HuH7.5 (RIG-I deficient) cells following SeV infection
at various multiplicities, assayed at 48 hours post-infection.
6E:2^dCt GAPDH-SEV (encoding Sendai virus genomic RNA) expression in HuH7 and HuH7.5 cell lines, assayed at 48 hours post-infection
6F:Fold change in INHBA expression, normalized to GAPDH, at 72 hours post-infection in HuH7.5.1 cells relative to uninfected (U.I.) cells.
Figure 6G:Fold change in MX1 expression, normalized to GAPDH, at 72 hours post-infection in HuH7.5.1 cells relative to uninfected (U.I.) cells.
7A:2^dCt Hprt-Inhba expression in the lungs of FLUAV PR/8 infected mice at 72 hours post-infection.
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7B:2^dCt Hprt-Isg15 expression in the lungs of FLUAV PR/8 infected mice at 72 hours post-infection.

7C:Correlation of Inhba and Isg15 transcript induction in the lungs of FLUAV PR/8 infected mice at 72 hours post-infection (linear regression, R2 = 0.9271, p=0.002)
7D:2^dCt Hprt-Inhba expression in the liver of FLUAV PR/8 infected mice at 72 hours post-infection

7E:2^dCt Hprt-Isg15 expression in the liver of FLUAV PR/8 infected mice at 72 hours post-infection
7F:2^dCt Hprt-Inhba induction in the lung tissue of FLUAV PR/8 infected mice at 48 hours post-infection, across a range of increasing multiplicities of infection.

7G:
2^dCt Hprt-Inhba expression in wildtype or MAVS-depleted mice at 48 hours post-infection with FLUAV PR/8

7H:
2^dCt Hprt-Isg15 expression in wildtype of MAVS-depleted mice at 48 hours post-infection with FLUAV PR/8

Supplementary Figure 1A:
Fold change INHBA normalized to GAPDH for HuH7 cells exposed to recombinant IFN or control medium (D10)

Supplementary Figure 1B/C/D:

OR6
cells incubated with a titration of IFNα in
the presence of titrating doses of activin A; assayed for Renilla luciferse and Cell Titer Glo activity.