Effect of CAR-M2 hydrogel treatment on the expression of related genes such as fibrosis and endothelial angiogenesis in mice with renal fibrosis

Kidney fibrosis involves the dynamic interactions of various cells, including immune cells, stromal cells, and ECs. Macrophages regulate regeneration and fibrosis through the interaction with other cell types, inducing some effects including the clearance of apoptotic myofibroblasts. Moreover, M2 macrophages promote tissue angiogenesis and participate in the reparative processes of soft tissue remodeling. Therefore, in addition to the direct effect of macrophage on renal fibrosis, its indirect regulatory role is also worthy to be explored. Its interaction with activated fibroblasts and ECs in renal fibrosis is unknown. Here our data discovered the heterogenicity and interaction of stroma and EC. Moreover, the infiltration of Cxcr2+ EC and expression of Lcn2 were indentfied highly related renal fibrosis. Taken together, our data provide a deeper understanding of the expression profile of renal fibrosis in mice, provide novel molecular targets for the treatment of renal fibrosis. Overall design: To investigate the effect of CAR-M2 on renal fibrosis expression genes, we set the Sham group, fibrosis model UUO group, and CAR-M2 treatment group for sequencing. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different groups at 14 days. Comparative gene expression profiling analysis of RNA-seq data for 3 groups.