Transcription profiling by array of two sub-clones of the SUIT2 pancreatic cancer cell line (S2-007 and S2-028) in order to identify metastasis-associated candidate genes

S2-007 and S2-028 are cell lines derived from the same primary pancreatic adenocarcinoma, but displying strong differences in their invasive and metastatic potential both in vitro and in vivo. Three independent preparations each of the highly metastatic S2-007 and the low metastatic S2-028 cell line grown in 2D cell culture (DMEM (Gibco, Invitrogen, Carlsbad, CA) supplemented with 10% (v/v) fetal bovine serum (PAA, Pasching, Austria) in a humidified atmosphere and 5% CO2, 95% air at 37°C) were analyzed by expression profiling.