Chromatin binding of RUNX1 in T-cell acute lymphoblastic leukemia (T-ALL) cells with depletion cBAF complex [ChIP-seq]

Using ChIP-seq of RUNX1 in the cells with cBAF-targeting gRNA, we identified RUNX1 binding chromain loci which is regulated by cBAF complex Cas9-expressing cells were lentivirally transduced with gRNA, selected with puromycin for 4 days, and then harvested on day 7 after gRNA transduction.