Long noncoding RNA expression levels in BXPC-3 cells under different nutritional conditions.. Long noncoding RNA expression levels in BXPC-3 cells under different nutritional conditions.

In order to explore the relationship between long non-coding RNA expression and EMT in pancreatic cancer under different nutritional conditions, we carried out Microarray-based gene expression analysis.BXPC-3 cells were cultured under three conditions: nutrient-sufficient, low glucose and low glutamine for 48 hours and then long non-coding RNA Microarray-based gene expression analysis was carried out to observe the changes in long non-coding RNA expression, so as to explore the specific mechanism of EMT in pancreatic cancer cells under nutritional stress conditions. Overall design: Microarray analysis for lncRNAs was conducted with BxPC-3 cells cultured with saturating nutrient medium, Glc- medium and Gln- medium for 48 h.