Primers used to amplify short fragments of the D-loop region.

The four overlapping fragments are indicated by the numbers in the first column. Species names are abbreviated as follows: R. argentiventer (arg), R. everetti (eve), R. mordax (mor), R. niobe (nio), R. nitidus (nit), R. praetor (pra), and R. verecundus (ver).*indicates the primer pairs for each fragment within each species column. The amplicon length (bp before trimming the primer) is indicated by the number in brackets after the forward primer the first time each pair occurs in the table. Primer names ending in L or F are forward primers and H or R are reverse primers. Fig. S1 shows the approximate positions of these primers.