Transcriptomic analysis of a Saccharomyces cerevisiae mutant with improved coniferyl aldehyde resistance

A Saccharomyces cerevisiae mutant with improved coniferyl aldehyde resistance was obtained by using an evolutionary engineering strategy, based on successive batch cultivation under gradually increased coniferyl aldehyde concentration. The mutant strain BH13 was selected which could grow at a coniferyl aldehyde concentration that the reference strain could not grow at all. Whole-genome transcriptomic analysis of BH13 with respect to the reference strain was performed to identify differences in gene expression levels between the two strains.本研究采用进化工程策略，通过在逐步升高的阿魏醛（coniferyl aldehyde）浓度条件下开展连续分批培养，成功获得一株耐阿魏醛能力提升的酿酒酵母（Saccharomyces cerevisiae）突变体。最终筛选得到突变菌株BH13，其可在对照菌株完全无法生长的阿魏醛浓度下正常生长。随后，本研究针对突变菌株BH13与对照菌株开展全基因组转录组分析，以鉴定二者在基因表达水平上的差异。