Transcriptome sequencing of Bactrocera and Zeugodacus fruit flies. Bactrocera and Zeugodacus

Development of novel molecular methods for accurate and economical identification of species has become critical both for pure biological research and in a wide range of applied areas. The most widely used current molecular diagnostic tool, the mitochondrial cytochrome c oxidase subunit 1 gene (CO1), the so-called ‘DNA barcode’, has been highly criticised and is known to be ineffective at distinguishing species in many groups. To address this, we used transcriptomes and a novel analytical workflow to develop new potential diagnostic markers.