Comprehensive analysis of H3K9me2 oocytes, together with DNA methylation and expression analysis of G9a conditional KO oocytes and maternal KO preimplantation embryos

We report the first genome-wide landscape of H3K9me2 ChIP-seq profile in mouse oocytes. We also performed whole-genome bisulfite sequencing and RNA-seq analysis of G9a conditional KO oocytes and maternal KO preimplantation embryos. Our findings illuminate the functional importance of G9a in preimplantation development and, in addition, pose a question on the proposed role for H3K9me2 in protection of the maternal genome from active CG demethylation.

本研究首次绘制了小鼠卵母细胞中H3K9me2染色质免疫共沉淀测序（ChIP-seq）的全基因组全景图谱。我们还对G9a条件性敲除卵母细胞与母源敲除植入前胚胎开展了全基因组亚硫酸氢盐测序及RNA测序（RNA-seq）分析。本研究结果阐明了G9a在植入前发育中的功能重要性，同时也对“已提出的H3K9me2可保护母源基因组免受活性CG去甲基化”这一功能假说提出了质疑。