Different forms of KLF5 regulate distinct sets of genes in prostate cancer cells [RNA-seq]

To identify which genes are regulated by KLF5 and its acetylation, we performed RNA-Seq and bioinformatics analyses in KLF5-null prostate cancer cells expressing KLF5, KLF5KR, and KLF5KQ. RNA-Seq analyses were performed using KLF5 knockout DU 145 and PC-3 cells that ectopically expressed empty vector (EV), wildtype KLF5 (KLF5); acetylation deficient mutant KLF5K369R (KR), and acetylation mimicking mutant KLF5K369Q (KQ).