First evidence of WW superfemale for hybrid sturgeon, the bester (Huso huso × Acipenser ruthenus)

Sex control is a promising approach for cost-effective aquaculture in certain fish species. Sturgeons possess ZZ/ZW genetic sex determination system and are popular aquaculture target for high-priced black caviar originated from female roe. Therefore, the production of all-female population is highly desired, but this has not yet been achieved to date. In this study, we identified a short nucleotide sequence specific to putative Z and W scaffolds of Acipenser ruthenus genome and developed the PCR to discriminate Z and W alleles. Using this PCR, A. ruthenus (N = 1,280), A. baerii baerii (N = 66), and F2 of the bester hybrid (Huso huso × A. ruthenus: N = 31) were segregated into dimorphic pattern of a single Z amplicon and dual Z plus W amplicons, with approximately 1:1 ratio. Of those, the gonadal sexes were identified for 145 individuals, and all were concordant with their genetic sex. Additionally, we applied the Z/W-discriminative PCR for gynogenetic population of F3 bester and identi..., , ,

性别控制是实现部分鱼类水产养殖降本增效的极具前景的技术路径。鲟鱼兼具ZZ/ZW型遗传性别决定系统与高经济价值：其雌性卵粒可制备高端黑鱼子酱，因此成为热门水产养殖物种。因此，全雌种群的规模化生产具有极高的应用需求，但截至目前尚未达成这一目标。本研究鉴定出裸腹鲟（Acipenser ruthenus）基因组中推定Z和W染色体基因组支架（scaffold）的特异性短核苷酸序列，并开发了可区分Z与W等位基因的PCR（聚合酶链式反应）检测技术。利用该PCR技术，我们对裸腹鲟（N=1280）、西伯利亚鲟西伯利亚亚种（A. baerii baerii，N=66）以及贝斯特杂交鲟（欧鳇Huso huso × 裸腹鲟A. ruthenus）的F2代个体（N=31）进行分型，结果显示样本可被分为两类二态性分型：仅携带单条Z等位基因扩增产物，以及同时携带Z与W等位基因的双扩增产物，两类个体的比例约为1:1。其中，我们对145个个体进行了性腺性别鉴定，所有个体的遗传性别均与性腺表型完全一致。此外，本研究将该Z/W鉴别PCR技术应用于贝斯特杂交鲟F3代的雌核发育群体，并进行了……