C. elegans RNA Sequence for viral discovery

Nucleic acid from two nematode species, C. elegans JU1580 (isolated from a rotting apple in Orsay, France) and C. briggsae JU1264 (isolated from a snail on a rotting grape in Santeuil, France) were prepared and pyrosequenced on the Roche Titanium Genome Sequencer. Raw sequence reads were filtered for quality and repetitive sequences as described in "Natural and experimental infection of Caenorhabditis nematodes by novel viruses related to nodaviruses" by F?©lix et al (http://www.ncbi.nlm.nih.gov/pubmed/21283608). Two novel members of the Nodaviridae viral family, Orsay nodavirus and Santeuil nodavirus, were discovered in the the 15933 and 20,787 reads, respectively. 1046 Orsay nodavirus reads were found in the C. elegans samples while 2492 Santeuil nodavirus reads were identified in in the C. briggsae sample. The data are provided in FASTA format.从两个线虫物种中提取核酸，分别为从法国奥尔赛腐烂苹果中分离的秀丽隐杆线虫（C. elegans）JU1580株，以及从法国桑特伊腐烂葡萄上的蜗牛中分离的布氏线虫（C. briggsae）JU1264株；随后对上述核酸进行制备，并采用罗氏钛金基因组测序仪（Roche Titanium Genome Sequencer）完成焦磷酸测序。原始序列读段参照Félix等人发表的论文《Natural and experimental infection of Caenorhabditis nematodes by novel viruses related to nodaviruses》（PubMed收录编号：21283608）中描述的方法，进行质量过滤与重复序列去除。研究人员分别在15933条秀丽隐杆线虫样本序列读段与20787条布氏线虫样本序列读段中，发现了诺达病毒科（Nodaviridae）的两个新成员：奥尔赛诺达病毒（Orsay nodavirus）与桑特伊诺达病毒（Santeuil nodavirus）。其中秀丽隐杆线虫样本中共检出1046条奥尔赛诺达病毒读段，布氏线虫样本中则鉴定出2492条桑特伊诺达病毒读段。本数据集以FASTA格式提供。