Dynamin-dependent entry of Chlamydia trachomatis is sequentially regulated by the effectors TarP and TmeA

Chlamydia invasion of epithelial cells is a pathogen-driven process involving two functionally distinct effectors – TarP and TmeA. They collaborate to promote robust actin dynamics at sites of entry. Here, we extend studies on the molecular mechanism of invasion by implicating the host GTPase dynamin 2 (Dyn2) in the completion of pathogen uptake. Importantly, Dyn2 function is modulated by TarP and TmeA at the levels of recruitment and activation through oligomerization, respectively. TarP-dependent recruitment requires phosphatidylinositol 3-kinase and the small GTPase Rac1, while TmeA has a post-recruitment role related to Dyn2 oligomerization. This is based on the rescue of invasion duration and efficiency in the absence of TmeA by the Dyn2 oligomer-stabilizing small molecule activator Ryngo 1-23. Notably, Dyn2 also regulated the turnover of TarP- and TmeA-associated actin networks, with disrupted Dyn2 function resulting in aberrant turnover dynamics, thus establishing the interdepend..., This repository contains spreadsheets compiling and analyzing the recruitment of proteins during Chlamydia invasion, quantification of Chlamydia invasion efficiency under various conditions, quantification of fixed-cell immunofluorescence, and dextran uptake data. Broadly speaking, data were obtained through fluorescence microscopy analysis of invading C. trachomatis and subsequent quantitative analysis of invasion-associated parameters., , # Dynamin-dependent entry of *Chlamydia trachomatis* is sequentially regulated by the effectors TarP and TmeA

Author Information:
Matthew D. Romero, Rey A. Carabeo
Department of Pathology and Microbiology, College of Medicine, University of Nebraska Medical Center, Omaha, NE
Corresponding Author Email: [[e-mail]](mailto:[e-mail])

Date of Collection: September 2022 - February 2024

Collected at: University of Nebraska Medical Center (2022-Current)

Project Funding: This study was supported by funding from the U.S. National Institutes of Health, and the National Institutes of Allergy and Infectious Disease grants R01 AI065545 (R.A.C.). M.D.R. was further supported by a fellowship from the Seattle Chapter of Achievement Rewards for College Scientists (ARCS).

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## Data and File Overview:

This repository contains spreadsheets compiling and analyzing the recruitment of proteins during *Chlamydia* invasion, quantification of *Chlamydia* invasion efficiency under ...