Analysis of the stress response after induction of the dormancy-inducing membrane toxin TisB in Escherichia coli

We assume that adaptation to tisB expression is driven by cellular stress responses. It has already been observed that expression of type I toxins leads to up-regulation of several stress-related genes. To reveal the response to TisB on a global scale, transcriptome analysis of MG1655 p0SD-tisB was performed. Wild type MG1655, harboring the p0SD-tisB plasmid, was treated with L-ara for tisB induction during exponential phase for 30 min. RNA samples of biological triplicates were extracted before (exp) and after L-ara treatment (0ATG), and after 1 hour (rec1h) and 2 hours (rec2h) of recovery.