Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for researching lychee downy blight (miRNA-Seq)

Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight . Overall design: The gene expression profiling analysis using data obtained from RNA-seq of 'Feizixiao'. The treated leaves and treated fruits were collected at 24 hpi. whole-transcriptome RNA sequencing was performed by Biomarker Technologies(Beijing, China) using an Illumina Nova platform.