豆科树木共生固氮生态生理及资源开发利用研究

该项目研究了我国南方29属65种豆科树种共生固氮能力和分布， 对其中一些优良树种进行了系统的共生固氮生态生理研究， 并在超微结构水平上研究了根瘤细胞结构与固氮功能的关系。提出只有基本结构与生理因素都达到成熟阶段的根瘤比例占优势时， 固氮活性才达最高值。报道了豆科树木根瘤类菌体周膜融合及其衰老的超微结构研究结果， 并提出周膜融合过程5个阶段的超微结构特征的见解。报道了大叶相思等豆科树木根瘤具有吸氢酶活性。应用银合欢叶取代米糠栽培食用菌获得成功。

This project investigated the symbiotic nitrogen fixation capacity and distribution patterns of 65 leguminous tree species across 29 genera in southern China. Systematic eco-physiological studies on symbiotic nitrogen fixation were conducted for several elite leguminous tree species from this cohort. Additionally, the correlation between nodule cellular structure and nitrogen fixation function was analyzed at the ultrastructural level. The study proposed that nitrogen fixation activity reaches its peak only when nodules with fully matured basic structure and physiological factors account for the majority. It reported the ultrastructural findings on the fusion and senescence of the peribacteroid membrane (PBM) in leguminous tree nodules, and proposed insights into the ultrastructural traits of the five stages of the PBM fusion process. The study also documented that nodules of leguminous tree species including Acacia auriculiformis exhibit uptake hydrogenase activity. Moreover, the successful cultivation of edible fungi using Leucaena leucocephala leaves as a substitute for rice bran was achieved.