DataSheet_1_A 13-LOX participates in the biosynthesis of JAs and is related to the accumulation of baicalein and wogonin in Scutellaria baicalensis.pdf

Although baicalein and wogonin contents in Scutellaria baicalensis, a traditional Chinese herb, are known to be regulated by jasmonic acid, the exact mechanism by which jasmonic acid regulates the accumulation of baicalein and wogonin remains unclear. In this study, we discovered SbLOX3, a gene encoding 13-lipoxygenase from the roots of S. baicalensis, which plays an important role in the biosynthesis of jasmonic acid. The contents of methyl jasmonate, baicalin, wogonin, and three metabolic intermediates of methyl jasmonate, 13-HPOT, OPDA, and OPC-8, were downregulated in the hair roots of the SbLOX3 RNAi lines. We confirmed that SbLOX3 was induced by drought stress simulated by PEG and Fusarium oxysporum, which subsequently led to changes in the content of MeJA, baicalin, and wogonin. Taken together, our results indicate that a 13-LOX is involved in the biosynthesis of jasmonic acid, and regulates the accumulation of baicalein and wogonin in S. baicalensis roots.

尽管从传统中药植物黄芩（Scutellaria baicalensis）中提取的黄芩苷和汉黄芩苷的含量已知受茉莉酸调控，但茉莉酸调节黄芩苷和汉黄芩苷积累的确切机制尚不明确。在本研究中，我们发现了黄芩中的SbLOX3基因，该基因编码13-脂氧合酶，主要位于其根部，并在茉莉酸的生物合成过程中发挥着关键作用。甲基茉莉酸、黄芩苷、汉黄芩苷以及甲基茉莉酸的三种代谢中间体（13-HPOT、OPDA和OPC-8）在SbLOX3 RNAi线毛根中的含量均受到下调。我们证实SbLOX3受PEG模拟的干旱胁迫和尖孢镰刀菌（Fusarium oxysporum）诱导，进而导致MeJA、黄芩苷和汉黄芩苷含量的变化。综合上述结果，我们的研究结果表明13-脂氧合酶参与了茉莉酸的生物合成过程，并调节了黄芩根部黄芩苷和汉黄芩苷的积累。