Additional file 3 of Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.)

Additional file 3: Figure S1. Gene ontology classification of the assembled unigenes. The Y-axis indicates the number of unigenes and X-axis indicates the GO categories. Figure S2 Functional classification of KEGG pathways of the assembled unigenes. The KEGG pathways were classified into six functional categories: A- Metabolism; B- Genetic Information Processing; C- Environmental Information Processing; D- Cellular Processes; E- Organismal Systems; F- Human Diseases. The Y-axis represents the KEGG metabolic pathways. The X-axis represents number of unigenes annotated in that particular pathway. Figure S3 Hierarchical tree graph of over-represented GO terms in the biological process category of down-regulated DEGS. Boxes in the graph represent GO terms labeled according to their GO ID, term definition, and statistical information. Significant terms (adjusted P ≤ 0.05) are in color (red, orange, or yellow), while non-significant terms are shown as white boxes. In the diagram, the degree of color saturation of a box is positively correlated with the enrichment level of the term. Solid, dashed, and dotted lines represent two, one, and zero enriched terms at both ends connected by the line, respectively. The rank direction of the graph is set from top to bottom. Figure S4 Hierarchical tree graph of over-represented GO terms in the biological process category of up-regulated DEGS. Boxes in the graph represent GO terms labeled according to their GO ID, term definition, and statistical information. Significant terms (adjusted P ≤ 0.05) are in color (red, orange, or yellow), while non-significant terms are shown as white boxes. In the diagram, the degree of color saturation of a box is positively correlated with the enrichment level of the term. Solid, dashed, and dotted lines represent two, one, and zero enriched terms at both ends connected by the line, respectively. The rank direction of the graph is set from top to bottom.

附加文件3：图S1。组装所得单基因（unigene）的基因本体（Gene Ontology, GO）分类。纵轴代表单基因数量，横轴代表GO分类类别。
图S2 组装所得单基因的KEGG（Kyoto Encyclopedia of Genes and Genomes）通路功能分类。KEGG通路被划分为六大功能类别：A-代谢（Metabolism）；B-遗传信息处理（Genetic Information Processing）；C-环境信息处理（Environmental Information Processing）；D-细胞过程（Cellular Processes）；E-生物系统（Organismal Systems）；F-人类疾病（Human Diseases）。纵轴代表KEGG代谢通路，横轴代表注释至该特定通路的单基因数量。
图S3 下调差异表达基因（differentially expressed genes, DEGs）生物过程类别中显著富集GO术语的层级树状图。图中方框代表依据其GO编号、术语定义及统计信息标注的GO术语。显著富集术语（校正后P≤0.05）以彩色（红、橙或黄）展示，非显著术语以白色方框呈现。该示意图中，方框颜色饱和度与术语富集程度呈正相关。实线、虚线与点线分别代表连线两端节点存在2个、1个和0个富集术语。该图排布方向为自上而下。
图S4 上调差异表达基因（differentially expressed genes, DEGs）生物过程类别中显著富集GO术语的层级树状图。图中方框代表依据其GO编号、术语定义及统计信息标注的GO术语。显著富集术语（校正后P≤0.05）以彩色（红、橙或黄）展示，非显著术语以白色方框呈现。该示意图中，方框颜色饱和度与术语富集程度呈正相关。实线、虚线与点线分别代表连线两端节点存在2个、1个和0个富集术语。该图排布方向为自上而下。