Effect of Exogenous Retinoic Acid on human iPSCs derived intestinal organoids. Effect of Exogenous Retinoic Acid on human iPSCs derived intestinal organoids

Retinoic acid promotes the in vitro growth, patterning and improves the cellular composition of human pluripotent stem-cell-derived intestinal organoids Overall design: 35-day old human intestinal organoids (HIO) generated from clump were collected for RNA-Seq. analysis. One group of HIO were cultured in basic gut medium with EGF (HIO control) and the other group of HIO were treated with EGF plus retinoic acid (HIO + RA).