Gene Expression Signature in Rat Pituitary MMQ Cells Treated With DRD2 agonist UNC9994

DRD2 agonists are effective in treating pituitary tumors. To fathom the β-arresin-dependent mechanisms underlying DRD2-mediated pituitary tumor growth suppression,we applied RNA-seq analysis to rat pituitary MMQ cells treated with a DRD2 β-arresin-biased agoinst, UNC9994.MMQ cells were treated with UNC9994(15μM) or vehecle control for 12 h or 24 h and then subject to RNA-seq analysis.We found oxidative-stress-related genes such as Nqo1 and Hmox1 were upregulated by UNC9994, revealing oxidative stress may be the basis of UNC9994-induced tumor growth suppression. Our study represents the first detailed analysis of transcriptomes in rat pituitary MMQ cells treated with DRD2 β-arresin-biased agonist.The significance of altered expression of specific transcripts will enhance our understanding of DRD2 signaling in pituitary tumor cells.多巴胺D2受体（DRD2）激动剂可有效治疗垂体肿瘤。为阐明DRD2介导的垂体肿瘤生长抑制所依赖的β抑制蛋白（β-arresin）相关机制，我们对经DRD2 β-arresin偏倚激动剂UNC9994处理的大鼠垂体MMQ细胞开展了RNA测序（RNA-seq）分析。将MMQ细胞以15μM的UNC9994或溶剂对照处理12小时或24小时，随后进行RNA-seq分析。我们发现，UNC9994可上调Nqo1、Hmox1等氧化应激相关基因，提示氧化应激可能是UNC9994诱导肿瘤生长抑制的分子基础。本研究是首个针对经DRD2 β-arresin偏倚激动剂处理的大鼠垂体MMQ细胞转录组的详细分析。特定转录本表达改变的研究意义，将有助于我们加深对垂体肿瘤细胞中DRD2信号通路的理解。