Expression data from hippocampus in mouse brain. Expression data from hippocampus in mouse brain

Our microarray results showed there were up-regulated 28 genes and down-regulated 29 genes, which related depression and inflammatory response such as cytokine-cytokine receptor interaction, chemokine signaling pathway, dopaminergic synapse, glutamatergic synapse, GABAergic synapse, cholinergic synapse, and serotonergic synapse. Among these genes, especially, higher hippocampal mRNA expression of transthyretin (Ttr), Zinc finger protein of the cerebellum 1 (Zic1), and Ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2) was found in kososan-administered defeated mice than water-administered defeated mice Overall design: DNA microarray analysis was conducted using isolated RNAs extracted from the hippocampus as described previously (Sasaki et al., 2017). Double-stranded cDNA was synthesized from 100 ng of total RNA with the GeneAtlas 3' IVT Express Kit (Affymetrix Inc., Santa Clara, CA, USA). Biotin-labeled amplified RNA (aRNA) was synthesized using the GeneChip 3´ IVT Express Kit (Affymetrix Inc.). Purified aRNA was fragmented using the GeneAtlas 3´ IVT Express Kit and hybridized for 16 h at 45 °C using the GeneChip Mouse Genome 430 PM microarray (Affymetrix Inc.). The chip was washed and stained in the Gene Atlas Fluidics Station 400 (Affymetrix Inc.), and the resulting image was scanned using the GeneAtlas Imaging Station (Affymetrix Inc.). Data analysis was performed using the Affymetrix expression console (http://www.affymetrix.com) and the online data tool DAVID (Database for Annotation, Visualization and Integrated Discovery, v6.8, National Institute of Allergy and Infectious Diseases, NIH, USA, https://david.ncifcrf.gov/) (Huang et al., 2007a; Huang et al., 2007b). Among the clustered genes, the genes that contain the gene ontology concerning depression and inflammatory response (e.g., cytokine-cytokine receptor interaction, chemokine signaling pathway, dopaminergic synapse, glutamatergic synapse, GABAergic synapse, cholinergic synapse, and serotonergic synapse) were selected using DAVID tool and manually.