Gene expression profiling of IRBIT mutant flies

In order to understand the biological role of Drosophila IRBIT, we generated null mutant fruitflies. We performed Illumina platform-based RNA-Seq analysis to investigate transcriptional consequences of the mutation using fly midguts as well as whole animals. We performed gene expression profiling on wildtype and IRBIT mutant flies. We prepared RNA-Seq libraries from midguts and whole flies. Flies were reared to be 1 day, 7 days, 40 days, or 50 days old. Males and females separately prepared under different treatments: normal, deoxyadenosine, deoxyguanosine, hydroxyurea. We also culture flies in two different conditions of normal versus axenic conditions. Experiments were triplicated for RNA-Seq except for 50 day-old samples. We generated, stranded, single-end RNA-Seq libraries to have 50 or 76 read-lengths.