Amphiregulin from regulatory T cells promotes liver fibrosis and insulin resistance in non-alcoholic steatohepatitis II. Amphiregulin from regulatory T cells promotes liver fibrosis and insulin resistance in non-alcoholic steatohepatitis II

We study the effect of Treg cell-derived Areg on hepatic stellate cells. Here we look at the transcriptional effect of Areg on hepatic stellate cells in vitro. Overall design: Quiescent hepatic stellate cells were isolated and cultured for 5 hours, then stimulated with recombinant mouse Areg (500 ng/mL) for 4 hours in starving media.