Hippo Pathway Deficiency in Gastric Pit Cells: A Spontaneous Mouse Model of Antral Carcinogenesis

Overview: Multi-omic profiling of a pit cell–targeted double-knockout (DKO) mouse model of antral gastric carcinogenesis and wild-type (WT) controls, all from gastric antrum tissue. Modalities include single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, bulk proteomics, and untargeted metabolomics with age-matched designs.scRNA-seq: DKO (12 mice at 3, 6, 9, and 12 months; three per age) pooled within age to generate four DKO libraries; WT tissues from four mice (one per age) were pooled across ages to produce a single control library.Spatial transcriptomics: Four samples from the contralateral antrum of the scRNA-seq cohort: one DKO and one age-matched WT at 6 months, and one DKO and one WT at 12 months.Proteomics: Twelve antral tissues across four groups (DKO4, DKO8, WT4, WT8; n=3 per group) for quantitative protein profiling.Metabolomics: Thirty-two antral tissues across the same four groups (n=8 per group). Untargeted acquisition was performed in both positive and negative ion modes.