Single cell RNAseq of mouse CD3+ Adipose Tissue T cells

Despite studies implicating adipose tissue T cells (ATT) in the initiation and persistence of adipose tissue inflammation, fundamental gaps in knowledge regarding ATT function impedes progress towards understanding how obesity influences adaptive immunity. We hypothesized ATT activation and function would have tissue-resident specific properties and that obesity would potentiate their inflammatory properties. We assessed ATT activation and inflammatory potential within mouse stromal vascular fraction (SVF).To assess intracellular signaling mechanisms responsible for ATT inflammation impairments, single-cell RNA sequencing of ATTs from epididymal adipose tissue was performed from male chow and high fat diet (60% for 12 weeks) fed mice. FACS sorted CD3+ cells from epididymal adipose tissue from male lean (n=2) and HFD fed (n=1) C57Bl/6 mice were analyzed by single cell RNA-seq (10X genomics)