Inhibitory kinetics of <i>Eg</i>KU-8 on bovine and canine trypsins.

ak2, KI and k2/KI were calculated from time course experiments (see Figures 4B and 4C) according to the fit to equation 4 of kobs versus [I] plots. Values are averages of independent measurements ± the standard error (n≥2). bk−2 were calculated from time course experiments according to equation 5. Values are averages of independent measurements ± the standard deviation (n = 15). cCalculated from equation 6 using the value of KI* determined in steady state inhibition experiments (Table 1). The value is the average of independent measurements ± the standard error (n = 2). dKI* were calculated from equation 6 using the values of k2, KI and k−2 obtained from time course experiments. The values are averages of independent measurements ± the standard error (n≥3). eND, not determined.