Additional file 1 of Tanshinone IIA attenuates neuroinflammation via inhibiting RAGE/NF-κB signaling pathway in vivo and in vitro

Additional file 1: Figure S1. Demonstration of Aβ1-42 oligomers by dot blot and electron microscopy a Dot blot analysis of the composition of Aβ1-42. 1 uL of Aβ1-42 was applied to a nitrocellulose membrane and probed with rabbit anti-oligomer antibody or with anti-amyloid fibril antibody. b Electron microscopy analysis of the structure of the Aβ1-42 aggregates. 20 uL of Aβ1-42 oligomer preparation was dropped onto a 300-mesh carbon nickelgrid, and after 5 min the solution was removed. Sample was stained for 2 min with phosphotungstic acid. The diameter of preparation is consistent with that of the oligomer diameter distribution.

附加文件1：图S1。通过斑点印迹（dot blot）与电子显微镜（electron microscopy）展示Aβ1-42寡聚体。a 斑点印迹分析Aβ1-42的组分：取1 μL Aβ1-42样品点于硝酸纤维素膜上，采用兔抗寡聚体抗体或抗淀粉样蛋白纤维抗体进行免疫探测。b 电子显微镜分析Aβ1-42聚集体的结构：将20 μL Aβ1-42寡聚体制备液滴加至300目碳包被镍网上，静置5分钟后吸除残留溶液，以磷钨酸染色2分钟。该制备物的粒径与寡聚体粒径分布一致。