H5N8 Influenza A Virus Genomes: Codon Usage Bias and Spatiotemporal Distribution n = 5219

This dataset features <b>5,219</b> complete <b>H5N8</b> influenza A virus sequences from NCBI, processed through local bioinformatics workflows. Sequences were filtered for completeness, aligned with MAFFT (--parttree --retree 1), and trimmed. Codon-level metrics including ENc, CAI (human and avian), GC3, RSCU, CPB, and PR2 were computed per sequence. Collection year and country were parsed from headers. Includes aligned FASTA, codon metrics CSV, QC summaries, and publication-ready visualizations.CODON USAGE SUMMARY: H5N8========================================Total sequences: 5,219Valid CDS: 1,703 (32.6%)Valid CAI: 1,703 (32.6%)<br>METRIC STATISTICS (Mean ± SD): ENc (Effective Number of Codons): 35.697 ± 1.497 CPB (Codon Pair Bias): 5.186 ± 1.103 CAI_human: 3.416 ± 0.137 CAI_avian: 3.436 ± 0.141 GC3: 0.449 ± 0.031 PR2_A (A3/(A3+T3)): 0.564 ± 0.043 PR2_G (G3/(G3+C3)): 0.531 ± 0.032<br>INTERPRETATION: → Strong codon usage bias (ENc &lt; 40) → Similar adaptation to human/avian hostsData processed by: [e-mail] for upcoming journal Article.<br>