Gene expression signatures in anoikis-resistance of human prostate PC-3 cells

To investigate potential molecular targets that regulate metastasis and metabolism in anoikis-resistance of prostate cancer cells, we have establised anoikis-resistant prostate PC-3 cells with ultra-low attachment 6-well plates (Corning) and employed whole genome microarray expression profiling as a discovery platform to identify differentially expressed genes between anoikis-resistant PC-3 cells and corresponding parental cells. Prostate cancer PC-3 cells were continuously suspension cultured in ultra-low attachment 6-well plates for 7 days then transferred into normal plates with attachment culture for 2 days, and the re-adherent cells were regarded as anoikis-resistant. Three independent experiments were performed. Total RNA samples extracted from three independent anoikis-resistant cell samples and corresponding parental samples were applied for genome microarray expression profiling.