Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 18/10/2005 to 23/10/2005 (Hobart - Dumont d'Urville)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 18/10/2005 to 23/10/2005 on a trip from Hobart to Dumont D'Urville.Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于“拉斯特罗号（RV L'Astrolabe）”考察船（平台代码：FHZI），采集时段为2005年10月18日至2005年10月23日，航线为从霍巴特前往迪蒙·迪尔维尔站。
光系统II（Photosystem II，缩写PSII）的最大光化学效率，亦称PSII最大量子产额（Fv/Fm），是浮游植物研究中应用最为广泛的荧光参数之一。该参数代表潜在光化学效率，即光合装置捕获的光能用于光化学反应的概率。研究表明，Fv/Fm对物理化学性质的变化具有瞬时响应，可作为浮游植物整体健康状态或生理活性的诊断指标。将其与PSII吸收截面面积、叶绿素浓度相结合，可用于初级生产力测定（Cheah等，2011，《深海研究》）。
以船舶清洁海水管路持续供给3米深度的海水。在暗适应时长不低于15分钟的状态下，以1分钟为间隔连续测定快速重复率荧光（FRR）产额；测定所用闪光序列包含两组闪光：100个亚饱和微闪（闪光时长1.1 μs，闪间间隔2.8 μs）以及20个弛豫微闪（闪光时长1.1 μs，闪间间隔51.6 μs）。