Transcriptome analysis of human pancreatic preadipocytes and in vitro differentiated adipocytes

The aim of this study is to investigate the impact of the metabolic status on the transcriptome of isolated preadipocytes and in vitro differentiated adipocytes. We identified 38654 transcripts in pancreatic fat cells. We report that preadipocyte differentiation increased the abundance of mRNA levels of proteins related to adipogenesis and lipid metabolism. These changes in the transcriptome were absent or less pronounced in fat cells obtained from patients with prediabetes and type 2 diabetes. Meanwhile, mRNA levels of proteins involved in excellular matrix remodeling, angiogenesis and cytoskeleton organization were less abundant in adipocytes of all metabolic groups. Overall design: Isolated preadipocytes and respective in vitro differentiated adipocytes from 12 different human donors stratified between non-diabetic, prediabetic and type 2 diabetic metabolic status.