Deduced genomic structure of EM93.

The 1.42 million genotyping calls generated by hybridizing 480 segregants (120 tetrads) to custom Agilent genotyping arrays were used to determine the order and organization of the two heterozygous marker-types, S288C-like or non-S288C. Across each chromosome, the nearest marker-type was determined by linkage analysis. This provides a deduced genomic organization of EM93 and with information regarding the size and distribution of S288C-like and non-S288C regions across the EM93 genome. To clarify and to enhance the genomic overview, only the non-S288C marker type (black-bars) for both homologous chromosomes (1 and 2) is shown. Position of the rDNA is indicated with a (*). Regions without markers are homozygous, based on Tiling Array hybridization, and are mostly, if not entirely, S288C-like.