Hyperbaric oxygenation on adhesions prevention after laparotomy in rats

Abstract Purpose: To evaluate the effects of hyperbaric oxygenation on prevention of adhesions in the abdominal cavity after laparotomy. Methods: Fifty four rats underwent laparotomy; stitches were made in the four quadrant parietal peritoneum and abdominal cavity closure. Animals were divided into three groups: 1 - control; 2 - subjected to high pressures and oxygenation; 3 - subjected to 100% hyperbaric oxygenation. The animals in groups 2 and 3 were daily submitted to oxygenation hyperbaric chamber after surgery. On the seventh day another laparotomy, registration of procedure, assessment of adhesions and biopsies of the peritoneum were held. Professionals analyzed the videos and the biopsies. Results: Peritoneal cavity adhesions occurred in animals of three groups with no difference between them. In Group 3, the adhesions presented more fragile and vascular proliferation more pronounced, and there was no difference in comparison with the first and second groups. However, there was no significant difference in the evaluation of these parameters between the animals in groups 1 and 2. Conclusions: Postoperative hyperbaric oxygenation in rats submitted to laparotomy did not alter the frequency, but reduced the density of adhesions in the peritoneal cavity and promoted vascular proliferation. The change in atmospheric pressure alone had no influence on the results.

摘要
目的：评估高压氧（hyperbaric oxygenation）对开腹术后腹腔粘连的预防效果。
方法：54只大鼠接受开腹手术，于四象限壁腹膜处缝合后关闭腹腔。将实验动物分为3组：1组为对照组；2组接受高压环境联合氧疗；3组接受100%浓度高压氧疗。2组与3组的大鼠于术后每日送入高压氧舱接受治疗。术后第7天再次行开腹术，记录手术操作、评估腹腔粘连程度并获取腹膜活检标本。由专业人员对手术录像及活检标本进行分析。
结果：三组大鼠均出现腹腔粘连，组间粘连发生率无显著差异。3组的粘连组织质地更脆弱，血管增殖更为显著，但该组与1、2组相比，上述指标无统计学差异；此外，1组与2组间的各项评估参数亦无显著差异。
结论：对接受开腹术的大鼠实施术后高压氧疗，未改变腹腔粘连的发生率，但可降低粘连密度并促进血管增殖；仅改变大气压本身对实验结果无显著影响。