Table 2 in Discovery of Cytospora species associated with canker disease of tree hosts from Mount Dongling of China

Table 2. Genes used in this study with PCR primers, primer DNA sequence, optimal annealing temperature and corresponding references.
LocusDefinitionPrimersPrimer DNA sequence (5'-3')Optimal annealing temp (°C)References of primers usedITSinternal transcribed spacer of ribosomal RNAITS1TCCGTAGGTGAACCTGCGG51White et al. 1990ITS4TCCTCCGCTTTTGATATGCLSUlarge subunit of ribosomal RNALRORACCCGCTGAACTTAAGC55Vilgalys and Hester 1990LR7TACTACCACCAAGATCTactactinACT-512FATGTGCAAGGCCGGTTTCGC61Carbone and Kohn 1999ACT-783RTACGAGTCCTTCTGGCCCATrpb2RNA polymerase II second largest subunitRPB2-5FGA(T/C)GA(T/C)(A/C)G(A/T)GATCA(T/C)TT(T/C)GG52Liu et al. 1999RPB2-7cRCCCAT(A/G)GCTTG(T/C)TT(A/G)CCCATtef-1αtranslation elongation factor 1-alphaEF1-668FCGGTCACTTGATCTACAAGTGC55Alves et al. 2008EF1-1251RCCTCGAACTCACCAGTACCGtub2beta-tubulinBt2aGGTAACCAAATCGGTGCTGCTTTG55Glass and Donaldson 1995Bt2bACCCTCAGTGTAGTGACCCTTGGC