Expression data from Hela cells stably expressing the wild-type HBx and the four mutant HBx

In large cohort studies, several HBx mutants were identified as independent risk factors for HCC risk.In order to study the carcinogenic mechanism between different mutants, HeLa cell lines expressing wild type and different mutated HBx proteins were constructed. We used microarrays to describe gene expression in HeLa cells stably expressing the wild-type HBx and the four mutant HBx, and to identify distinct classes of up-regulated genes. HBV DNA extracted from HCC patients was used as a template to amplify HBx gene fragments and construct corresponding lentiviruses. Lentiviral vectors expressed different HBx mutant proteins in Hela cells. Microarray analysis was performed on the constructed cells.