Morphological, molecular and functional cardiac study during aging in animals submitted to gestational protein restriction

Purpose: In this study, we used RNA-Seq to characterize differences in the Left ventricle expression profiles rats with 62-week old submited to gestational protein restricion (LP) compared to normal protein (NP) offspring during de gestacional. Methods: Total RNA was extracted from left heart (rats with 62-week old submited to gestational protein restricion (LP) (n=5) compared to normal protein (NP) offspring during de gestacional) (n=5) using the Trizol methods. RNA concentration was quantitated using the NanoDrop. A library was built for each sample, using the TruSeq Stranded mRNA kit (illumina) and submitted to sequencing. Results: By genomic sequencing analysis, we verified changes in the expression of 137 genes; After gene to gene biological evaluation and relevance, the present study demonstrated significant differences in genes linked to inflammatory activity, oxidative stress, apoptosis process, autophagy and, in hypertrophy and fibrosis pathways resulting heart function disorders. Conclusion: The present study suggests that maternal protein restriction may lead to early heart disorders in the LP offspring compared to the NP group. We may hypothesize that rapid heart dysfunction is associated with heart fibrosis, myocardial cell hypertrophy and multiple gene expression abnormalities in the LP offspring. Overall design: Left ventricle mRNA profiles of Wistar Rat